CASP3F

Caspase 3 Assay Kit, Fluorimetric

• Synonym(s): Caspase-3 Activity Fluorometric Kit
• EC Number: 247-454-8
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 1,000 multiwell tests; sufficient for 100 standard tests (2 ml)
• Quality Level: 200
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CASP3(836)

Description

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Kit Components Only

• Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

• A1086 Ac-DEVD-AMC substrate 2.5 mg
• A0835 Ac-DEVD-CHO inhibitor .5 mg
• A9891 7-Amino-4-methylcoumarin standard 1 mg

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3