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CAS9BST

Sigma-Aldrich

CAS9 Blasticidin Lenti Plasmid

NACRES:
NA.51

Quality Level

recombinant

expressed in E. coli

packaging

vial of 50 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

selection

ampicillin
blasticidin

shipped in

dry ice

storage temp.

−20°C

General description

This product is a blasticidin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma′s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma′s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link below to order gRNA.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Components

1ug of plasmid DNA, EF1a-Cas9-2A-Blasticidin Lenti

Linkage

Click here to order gRNA plasmid and virus.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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