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C9499

Sigma-Aldrich

Cellulase, thermostable from Clostridium thermocellum

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase

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About This Item

CAS Number:
9012-54-8
Enzyme Commission number:
3.2.1.4 (BRENDA, IUBMB)
MDL number:
MFCD00081510
UNSPSC Code:
12352204

recombinant

expressed in E. coli

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

liquid

specific activity

≥40 units/mg protein

mol wt

41 kDa

concentration

19-21 mg protein/mL (UV)

shipped in

wet ice

storage temp.

2-8°C

Application

Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries . Cellulase is added to detergents to improve cleansing properties . Cellulase, product C9499, is from Clostridium thermocellum and is recombinant and expressed in E. coli.

Biochem/physiol Actions

Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites . Cellulases have different microbial origins and therefore different protein sequences . Product C9499 exhibits endo-cellulase, β-glucanase activity when assayed using insoluble AZCL-linked substrates as well as exo-cellulase and cellobiohydralase activities.

Unit Definition

One unit will produce 1 μmole of reducing sugar (measured as glucose) from β-glucan per minute at pH 5.8 at 70 °C.

Physical form

Supplied as as solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C9499-BULK:
C9499-2MG:
C9499-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gaku Tokuda et al.
Biology letters, 3(3), 336-339 (2007-03-22)
The intestinal flagellates of termites produce cellulases that contribute to cellulose digestion of their host termites. However, 75% of all termite species do not harbour the cellulolytic flagellates; the endogenous cellulase secreted from the midgut tissue has been considered a
A Brant et al.
Occupational and environmental medicine, 61(9), 793-795 (2004-08-20)
Three employees from two different detergent companies were investigated for occupational asthma, using skin prick tests, serum specific IgE, and specific bronchial challenge. Two were challenged with lipase and one with cellulase. All three cases had immunological evidence of sensitisation
Qiuhong Niu et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 52(11), 1408-1414 (2013-02-07)
To screen, identify bacterial strains with high capability to degrade cellulose from bacteria associated with Bursaphelenchus xylophilus and to clone related genes. First, we collected B. xylophilus samples from pine wood nematode disease areas in Nanyang, Henan province, China. Then
Yohei Yamakawa et al.
Biochemical and biophysical research communications, 431(4), 777-782 (2013-01-23)
Cellobiose triggers the production of two endoglucanases, EglA and EglB, in Aspergillus nidulans. The cellulose responsive element (CeRE) cis-element that is essential for induction has been identified on the eglA promoter, but transcription factors that bind to CeRE have not
Tomonori Nakai et al.
Journal of bacteriology, 195(5), 958-964 (2012-12-18)
Cellulases are enzymes that normally digest cellulose; however, some are known to play essential roles in cellulose biosynthesis. Although some endogenous cellulases of plants and cellulose-producing bacteria are reportedly involved in cellulose production, their functions in cellulose production are unknown.
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