C8831

Anti-Cyclin B₁ antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-CCNB
• MDL number: MFCD00243104
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 62 kDa
• species reactivity: human, mouse, hamster
• technique(s): indirect immunofluorescence: 1:30; microarray: suitable; western blot: 1:100
• UniProt accession no.: P14635
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CCNB1(891) ; mouse ... Ccnb1(268697)

Description

General description

The cyclin B1 (CCNB1) gene is mapped to human chromosome 5q13.2. The gene codes for a 62kDa protein containing a classical cyclin box domain. CCNB1 shuttles between the nucleus and cytoplasm until prophase begins. It is a member of the cyclin family and is expressed at low levels in normal cells.

Immunogen

recombinant hamster cyclin B₁

Application

Anti-cyclin B1 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Cyclin B1 (CCNB1) and cyclin-dependent kinase 1 (CDK1) complex play a crucial role in regulation of mitosis. Elevated expression of the gene has been observed in various types of human cancers including breast, lung, colon, prostate and head and neck cancers. Thus, this protein can be considered as a potent target for cancer vaccines. In addition, CCNB1 also serves as a prognostic biomarker for estrogen-receptor-positive (ER⁺) breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable