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C8740

Sigma-Aldrich

CelLytic™ B Cell Lysis Reagent

For bacterial cell lysis, 10× concentrate

Synonym(s):

bacterial cell lysis reagent

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About This Item

MDL number:
MFCD02097909
UNSPSC Code:
41116134
NACRES:
NA.56

Quality Level

200

form

liquid

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Application

A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
TM[="CelLytic"] B Cell Lysis Reagent has been used for lysing E.coli 5-helix bundle protein. It is also suitable for lysing hepatitis B X-interacting protein (HBXIP) and E. coli TG1 cells.

Features and Benefits

  • Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
  • Scalable for 1 to 25 grams of bacterial cell paste
  • No interference with downstream applications such as affinity chromatography, IP, and Western blotting
  • Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents

Other Notes

10X concentrate of detergent blend contains no buffering components, giving the researcher flexibility to integrate the lysis reagent into their desired system. Also amenable to automation protocols for recombinant protein extraction and purification.

Legal Information

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C8740-BULK:
C8740-100ML:
C8740-50ML:
C8740-VAR:
C8740PROC:
C8740-10ML:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ8409
SLCH2916
SLCF6936
SLCB8923
SLBW8423

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A fluorescence polarization assay using an engineered human respiratory syncytial virus F protein as a direct screening platform
Park M, et al.
Analytical Biochemistry, 409(2), 195-201 (2011)
Interaction of hepatitis B viral oncoprotein with cellular target HBXIP dysregulates centrosome dynamics and mitotic spindle formation
Wen Y, et al.
The Journal of biological chemistry, 283(5), 2793-2803 (2008)
A highly efficient procedure for the extraction of soluble proteins from bacterial cells with mild chaotropic solutions
Danilevich VN, et al.
Chemical Engineering & Technology, 31(6), 904-910 (2008)
Yihong Guan et al.
Communications biology, 3(1), 493-493 (2020-09-09)
Loss-of-function TET2 mutations (TET2MT) are common in myeloid neoplasia. TET2, a DNA dioxygenase, requires 2-oxoglutarate and Fe(II) to oxidize 5-methylcytosine. TET2MT thus result in hypermethylation and transcriptional repression. Ascorbic acid (AA) increases dioxygenase activity by facilitating Fe(III)/Fe(II) redox reaction and

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