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Safety Information

C6995

Sigma-Aldrich

Anti-COMT (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Catechol-O-methyltransferase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~24 kDa (and ~28 kDa)

species reactivity

human, rat, mouse

technique(s)

western blot: 1:250-1:500 using whole extracts of mouse and rat liver and HEK-293T cells expressing human COMT

UniProt accession no.

P21964

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... COMT(1312)
mouse ... Comt1(12846)
rat ... Comt(24267)

General description

The gene Catechol-O-methyltransferase (COMT) is mapped to human chromosome 22q11.2. COMT is also referred to as HEL-S-98n. It is widely expressed in various tissues, including brain, liver, kidney, endometrium and breast.

Specificity

The antibody recognizes MB-COMT and S-COMT.

Application

Anti-COMT (N-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Catechol-O-methyltransferase (COMT) is a Mg2+-dependent enzyme. COMT catalyzes transfer of methyl groups from S-adenosyl methionine to catecholamines. It is responsible for degradation of catecholamines and thereby results in psychiatric disorders and symptoms. Met/Met genotype individuals can acquire skill in executive function tasks (as multi-unmanned vehicles supervisory control, to a higher level and/or faster than other genotype groups). On the other hand, COMT polymorphism is linked to major depressive disorder and bipolar disorder. The genetic variants of COMT alter neurobehavioral functions that are affected by mercury in adults and might modify the adverse neurobehavioral effects of mercury exposure in children. COMT polymorphism is associated with high risk of premature ovarian insufficiency. COMT single nucleotide polymorphism preoperative humans psychological status and postoperative opioid analgesia efficacy. COMT acts as a tumor supressor gene for colorectal cancer cell lines. Overexpression of COMT supresses pancreatic cancer.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C6995-25UL:
C6995-200UL:
IXO14775:
C6995-VAR:
C6995-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Emerson Barchi Cordts et al.
Journal of ovarian research, 7, 47-47 (2014-05-09)
Estrogens are important factors in the female reproductive functions and are processed by a number of enzymes along their metabolic pathway. The COMT gene constitutes a crucial element in estrogen metabolism and is assumed to be involved in the development
Pituitary adenylate cyclase-activating polypeptide (PACAP) has a neuroprotective function in dopamine-based neurodegeneration in rat and snail parkinsonian models
Maasz G, et al.
Disease models & mechanisms, 10(2), 127-139 (2017)
A L Potapov et al.
Anesteziologiia i reanimatologiia, 60(3), 48-51 (2015-09-30)
To evaluate the influence of µl-opioid receptor (OPRM1) 118A>G and catechol-O-methyltransferase (COMT) 1947G>A gene single nucleotide polymorphisms (SNP) combinations on postoperative opioid analgesia (POA) efficacy. In 100 consecutive patients scheduled for major urologic surgeries the SNPs of OPRM1 118A>G and
G Pandolfo et al.
Journal of affective disorders, 177, 108-113 (2015-03-15)
Major depressive disorder (MDD) and bipolar disorder (BD) are the two most common mood disorders. Given the recognized involvement of catecholamines in depression, genetic research focused on the evaluation of polymorphisms in genes coding for proteins that regulate neurotransmitter release
Wenming Wu et al.
Archives of medical research, 46(1), 17-23 (2014-12-24)
Catechol-O-methyltransferase (COMT) has been reported as an important molecule in various types of cancers. The biological function of COMT in colorectal cancer (CRC) has not yet been fully investigated. We constructed a transient transfection of a CRC cell lines to
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