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Safety Information

C5572

Sigma-Aldrich

Click′s Medium

With sodium bicarbonate, without mercaptoethanol and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Click’s EHAA Medium, Eagle’s Ham’s amino acids; EHAA

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: yes
glucose: yes

shipped in

ambient

storage temp.

2-8°C

Application

Click′s Medium has been used:
  • to incubate cartilage discs to measure the ability of chondrocytes to synthesize sulphated glycosaminoglycans (GAGs) by a metabolic assay
  • as a culture medium to incubate frozen peripheral blood mononuclear cells (PBMCs) in vitro stimulation and evaluation of immunologic response
  • for the suspension, washing and culture of T-cells

Reconstitution

Supplement with 0.584 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C5572-BULK:
C5572-100ML:
C5572-VAR:
C5572-500ML:
C5572-6X500ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients.
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Clinical cancer research : an official journal of the American Association for Cancer Research, 23(13), 3352-3364 (2017-01-12)
David E Pegg et al.
Cryobiology, 93, 12-17 (2020-04-25)
Although it is relatively straightforward to cryopreserve living isolated chondrocytes, at the present time there is no satisfactory method to preserve surgical grafts between the time of procurement or manufacture and actual use. In earlier papers we have established that

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