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Key Documents

Safety Information

C4483

Sigma-Aldrich

CD152/Fc Chimera, Cytolytic from mouse

recombinant, expressed in NS.1 cells, buffered aqueous solution

Synonym(s):

CTLA4/Fc Chimera, cytolytic

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About This Item

MDL number:
MFCD01092888
UNSPSC Code:
51111800
NACRES:
NA.77

biological source

mouse

Quality Level

200

recombinant

expressed in NS.1 cells

Assay

≥99% (SDS-PAGE)

form

buffered aqueous solution

mol wt

97 kDa

packaging

pkg of 1 mg

storage condition

avoid repeated freeze/thaw cycles

impurities

≤1 EU/mg protein Endotoxin level (LAL test)

UniProt accession no.

P09793

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Cd152(12477) , Ctla4(12477)

General description

The complement (C1q) and FcγR I binding sites of the Fcγ2a fragment allow the Fc portion of the fusion protein to effectively facilitate direct antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Biochem/physiol Actions

CD152 (CTLA4), a cell surface glycoprotein expressed at low levels on activated T cells, is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). A related cell surface glycoprotein, CD28, binds to CD80 and CD86 with lower affinity. The soluble CD152/Fc chimeric fusion protein blocks the B7/CD28 signaling pathway by binding to CD80 and CD86.

Other Notes

The extracellular domain (160 amino acids) of mouse CD152 is fused to mouse IgG2a Fc domain.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4, with no preservative added.

Preparation Note

Purified from serum-free tissue culture supernatant

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C4483-VAR:
C4483-1MG-PW:
C4483-1MG:
C4483-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
040M1664
099K1069
059K1322
049K1509
118K1200

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Amod A Sarnaik et al.
Cancer journal (Sudbury, Mass.), 15(3), 169-173 (2009-06-27)
Metastatic melanoma is a disease associated with poor prognosis, with a median survival reported to range from 6 to 9 months. Patients who are not candidates for surgical resection have an even worse expected survival. This is largely due to
W Steurer et al.
Journal of immunology (Baltimore, Md. : 1950), 155(3), 1165-1174 (1995-08-01)
To test the hypothesis that blockade of B7-triggered costimulation by donor cells could preclude allograft rejection, we coated crude islet allograft preparations in vitro for 1 h with a murine CTLA4/Fc fusion protein. Murine CTLA4/Fc blocks the proliferative response in
Andrew L Mellor et al.
International immunology, 16(10), 1391-1401 (2004-09-08)
Murine dendritic cells (DCs) expressing indoleamine 2,3 dioxygenase (IDO) catabolize tryptophan and can suppress T cell responses elicited in vivo. Here, we identify specific subsets of splenic (CD11c+) dendritic cells competent to mediate IDO-dependent T cell suppression following CTLA4-mediated ligation
Andrew L Mellor et al.
Journal of immunology (Baltimore, Md. : 1950), 171(4), 1652-1655 (2003-08-07)
In mice, immunoregulatory APCs express the dendritic cell (DC) marker CD11c, and one or more distinctive markers (CD8alpha, B220, DX5). In this study, we show that expression of the tryptophan-degrading enzyme indoleamine 2,3 dioxygenase (IDO) is selectively induced in specific
P S Linsley et al.
The Journal of experimental medicine, 174(3), 561-569 (1991-09-01)
Functional interactions between T and B lymphocytes are necessary for optimal activation of an immune response. Recently, the T lymphocyte receptor CD28 was shown to bind the B7 counter-receptor on activated B lymphocytes, and subsequently to costimulate interleukin 2 production

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