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C4405

Sigma-Aldrich

Choline Oxidase from Arthrobacter globiformis

lyophilized powder, 8-20 units/mg solid

Synonym(s):

Choline: oxygen 1-oxidoreductase

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About This Item

CAS Number:
9028-67-5
Enzyme Commission number:
1.1.3.17 (BRENDA, IUBMB)
EC Number:
232-840-0
MDL number:
MFCD00130787
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

specific activity

8-20 units/mg solid

mol wt

71 kDa by SDS-PAGE
 83 kDa by gel filtration

storage temp.

−20°C

General description

Research area: Cell signaling. Choline oxidaseis a flavoprotein and belongs to the glucose methanol choline(GMC)-oxidoreductase family.

Application

Choline Oxidase from Arthrobacter globiformis has been used:
  • for enzymatic determination of choline (Cho) and phosphocholine (P-Cho)
  • in sphingomyelin assay to determine the concentration of sphingomyelin by measuring free choline
  • in enzyme coating for the fully-assembled recording device for electrochemical measurements

Choline oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.Choline oxidase from Arthrobacterglobiformis is suitable for the preparation of amperometric biosensors.

Biochem/physiol Actions

Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5.

Unit Definition

One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.

related product

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C4405-BULK:
C4405-50UN:
C4405-100UN:
C4405-VAR:
C4405-500UN:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A multi-site array for combined local electrochemistry and electrophysiology in the non-human primate brain
Disney AA, et al.
Journal of Neuroscience Methods, 255, 29-37 (2015)
Choline import into chloroplasts limits glycine betaine synthesis in tobacco: analysis of plants engineered with a chloroplastic or a cytosolic pathway
Nuccio ML, et al.
Metabolic engineering, 2(4), 300-311 (2000)
Shihua Hou et al.
Biosensors & bioelectronics, 33(1), 44-49 (2012-01-11)
A novel acetylcholinesterase (AChE)/choline oxidase (ChOx) bienzyme amperometric acetylcholine biosensor based on gold nanoparticles (AuNPs) and multi-walled carbon nanotubes (MWCNTs) has been successfully developed by self-assembly process in combination of sol-gel technique. A thiolated aqueous silica sol containing MWCNTs and
Giovanni Gadda
Biochimica et biophysica acta, 1646(1-2), 112-118 (2003-03-15)
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme is capable of accepting betaine-aldehyde as a substrate, allowing the investigation of the reaction mechanism for both
Shengyuan Deng et al.
Biosensors & bioelectronics, 26(11), 4552-4558 (2011-06-10)
A signal amplification system for electrochemiluminescence (ECL) of quantum dots (QDs) was developed by using electrochemically reduced graphene oxide (ERGO) to construct a nanobiosensing platform. Due to the structural defects of GO, the ECL emission of QDs coated on GO
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