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Safety Information

B9553

Sigma-Aldrich

Monoclonal Anti-Bone Morphogenetic Protein 2 antibody produced in mouse

clone 65529.111, purified immunoglobulin, lyophilized powder

Synonym(s):

Anti-BMP-2

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About This Item

MDL number:
MFCD02095901
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

65529.111, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

western blot: 1-2 μg/mL

isotype

IgG2b

UniProt accession no.

P12643

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BMP2(650)

General description

Bone morphogenetic proteins (BMPs) are the members of the TGF-β superfamily and are identified as the critical factors for formation of bone and cartilage. Apart from this BMPs have immune regulatory functions. The cellular effects of BMPs are mediated by a heteromeric complex composed of type I and type II receptors. Among the BMPs, BMP-2 has bone and cartilage inducing abilities. The other important functions of BMP-2 are in heart formation. BMP-2 signaling also involves active cross talk with major pathways such as Smad, MAPK, PI3K and NF-κ B.. Monoclonal Anti-BMP-2 recognizes recombinant BMP-2. It also detects BMP-4 (25% cross reactivity).

Specificity

The antibody recognizes recombinant human BMP-2. It shows approximately 25% cross-reactivity with recombinant human BMP-4.

Immunogen

recombinant human BMP-2.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Monoclonal Anti-BMP-2 antibody may be used in immunoblotting at a working antibody concentration of 1-2 μg/ml.

Physical form

Lyophilizd from a 0.2 μm filtered solution in PBS, pH 7.4, with 5% trehalose.

Preparation Note

Purified using protein G.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

B9553-.5MG-PW:
B9553-BULK:
B9553-.5MG:
B9553-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Julia K Pester et al.
GMS Interdisciplinary plastic and reconstructive surgery DGPW, 2, Doc09-Doc09 (2013-01-01)
To determine bone morphogenetic protein (BMP)-2 protein and Aggrecan in osteoarthritic and healthy cartilage with special regard to localization and degree of cartilage damage 95 samples representing osteoarthritic cartilage and 17 samples out of normal cartilage were graded histological by
Suzanne N Lissenberg-Thunnissen et al.
International orthopaedics, 35(9), 1271-1280 (2011-06-24)
This review evaluates the application of bone morphogenetic proteins (BMPs) in delayed bone repair, aiming at a broad audience from clinicians to scientists. Next to an overview of the role of the different BMPs, their antagonists and their current applications
P C Bessa et al.
Journal of tissue engineering and regenerative medicine, 2(1), 1-13 (2008-02-23)
Discovered in 1965, bone morphogenetic proteins (BMPs) are a group of cytokines from the transforming growth factor-beta (TGFbeta) superfamily with significant roles in bone and cartilage formation. BMPs are used as powerful osteoinductive components of diverse tissue-engineering products for the
Kohei Miyazono et al.
Cytokine & growth factor reviews, 16(3), 251-263 (2005-05-06)
Bone morphogenetic proteins (BMPs), members of the transforming growth factor-beta (TGF-beta) superfamily, bind to two different serine/threonine kinase receptors, and mediate their signals through Smad-dependent and Smad-independent pathways. Receptor regulated-Smad (R-Smad) proteins specific for the BMP pathways interact with various
Flora Gilde et al.
Acta biomaterialia, 46, 55-67 (2016-09-17)
Surface coatings delivering BMP are a promising approach to render biomaterials osteoinductive. In contrast to soluble BMPs which can interact with their receptors at the dorsal side of the cell, BMPs presented as an insoluble cue physically bound to a

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