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Safety Information

B1310

Sigma-Aldrich

Anti-BACH1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-BRCA1-associated C-terminal helicase 1

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~150 kDa

species reactivity

human

technique(s)

immunoprecipitation (IP): 5-10 μg using RIPA extract (0.5 mg) of human HeLa cells
indirect immunofluorescence: 10-20 μg/mL using human MCF-7 cells
microarray: suitable
western blot: 0.5-1.0 μg/mL using whole extracts of MCF-7 human breast carcinoma cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BRIP1(83990)
mouse ... Brip1(237911)
rat ... Brip1(360588)

General description

BTB Domain And CNC Homolog 1 (BACH1) is expressed in numerous tissues. The protein includes a nuclear localization signal in its helical domain.

Immunogen

synthetic peptide corresponding to amino acid residues 1233-1249 of human BACH1 with N-terminal added cysteine, conjugated to KLH.

Application

Anti-BACH1 antibody produced in rabbit has been used in:
  • immunoblotting
  • immunohistochemistry
  • western blotting

Polyclonal anti-BACH1 antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10 μg by using RIPA extract (0.5 mg) of human HeLa cells and for indirect immunofluorescence at 10-20 μg/mL using human MCF-7 cells. The product is also suitable for western blot analysis at a working concentration of 0.5-1.0 μg/mL by using whole extracts of MCF-7 human breast carcinoma cells. Further, anti-BACH1 antibody has shown suitability for microarray analysis.

Biochem/physiol Actions

BACH1 interacts directly with the C-terminal BCRT repeats in Breast cancer type1 protein (BRCA1), a tumor suppressor nuclear phosphoprotein that facilitates the repair of double-stranded DNA breaks. Bach1 also functions as transcription repressor in transfection assays using fibroblast cells and as a transcriptional activator and repressor, respectively, in cultured erythroid cells. Additionally, it is also involved in the coordination of transcription activation and repression by Maf family members such as MafK. Bach1 (BTB and CNC homology 1, basic leucine zipper transcription factor 1) inhibits oxidative stress-inducible genes and is a negative regulator of oxidative stress-induced cellular senescence.
BTB Domain And CNC Homolog 1 (BACH1) interaction between breast cancer type 1 (BRCA1) in vivo appears to be dependent on phosphorylation of Ser990 in the latter during the G2/M phase of the cell cycle. BACH1 mutations were found in patients with early-onset breast cancer. It displays a punctate nuclear staining pattern similar to that observed for BRCA1.

Target description

BACH1, BRCA1 interacting protein C-terminal helicase 1, encodes a protein that is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand br

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

B1310-200UL:
B1310-VAR:
IXO11485:
B1310-BULK:


Certificates of Analysis (COA)

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Zhongjie Xu et al.
Oncology letters, 14(1), 345-348 (2017-07-12)
The present study aimed to investigate the association between breast cancer susceptibility gene 1 (BRCA1) expression and drug resistance in colon cancer, with the specific aim of elucidating the underlying molecular mechanisms of vincristine (VCR) resistance in tumor cells. The
BRCA1-mediated repression of mutagenic end-joining of DNA double-strand breaks requires complex formation with BACH1
Dohrn L, et al.
The Biochemical Journal, 441(3), 919-928 (2012)
Expression of the BRCA1-interacting protein Brip1/BACH1/FANCJ is driven by E2F and correlates with human breast cancer malignancy
Eelen G, et al.
Oncogene, 27(30), 4233-4233 (2008)
Kazuhiro Daino et al.
PloS one, 8(9), e74013-e74013 (2013-09-17)
BRIP1 is a DNA helicase that directly interacts with the C-terminal BRCT repeat of the breast cancer susceptibility protein BRCA1 and plays an important role in BRCA1-dependent DNA repair and DNA damage-induced checkpoint control. Recent studies implicate BRIP1 as a
The BRCT domain is a phospho-protein binding domain
Yu X, et al.
Science, 302(5645), 639-642 (2003)

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