AL11430
DMT-locA(bz) Phosphoramidite
configured for ABI
About This Item
Recommended Products
biological source
non-animal source (BSE/TSE no Risk)
Quality Level
product line
Proligo Reagents
Assay
≥98.0% (31P-NMR)
≥98.0% (reversed phase HPLC)
form
powder
impurities
≤3 wt. % Residual Solvent Content
<0.4% Water Content (Karl Fischer)
<0.5% Single unspecified Impurity (reversed phase HPLC)
color
white to off-white
solubility
soluble, clear, colorless
absorption
<0.1 in acetonitrile at 0.2 M
suitability
conforms to structure for H-NMR
conforms to structure for LC-MS
compatibility
configured for ABI
storage temp.
−20°C
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General description
With the exception of the LNA monomers, LNA synthesis is accomplished with the same reagents as DNA synthesis. LNA phosphoramidites from Merck are diluted with dry acetonitrile, except for locMeC(bz)-phosphoramidite. This phosphoramidite requires the application of a cosolvent to prevent crystallization from the solution on the synthesizer. Dichloromethane or tetrahydrofuran (THF) can be applied as co-solvents with acetonitrile to completely dissolve locMeC(bz)-phosphoramidite.
Features and Benefits
- LNA oligonucleotides are prepared by phosphoramidite chemistry
- Standard DNA synthesizer platforms can be employed. No change is required in the reagents commonly used for DNA synthesis
- To further enhance the hybridization characteristics of LNA, 5-methyl-cytidine is employed instead of cytidine
- LNA monomers are as soluble in acetonitrile as their DNA counterparts (except for the 5-methyl-cytidine derivative, which requires the application of 10-20%, dichloromethane as a co-solvent)
- Mixmer oligonucleotides containing LNA, DNA and/or RNA monomers can be assembled easily
- LNA oligonucleotides with predefined melting temperatures (Tm) can be designed and prepared
Other Notes
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
PDSCL
Deleterious substance
JAN Code
AL11430-1G:4548173351858
Certificates of Analysis (COA)
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