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Safety Information

A9253

Sigma-Aldrich

L-Amino Acid Oxidase from Crotalus adamanteus

Type I (dried venom)

Synonym(s):

L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Crotalus adamanteus

Quality Level

type

Type I (dried venom)
Type I

form

powder

mol wt

~130 kDa

solubility

H2O: soluble 1.0 mg/mL, clear(lit.)

storage temp.

−20°C

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General description

L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa. Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules. LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney. In the N-terminal region, it has a βαβ domain with glutamic acid residues. LAAO imparts yellow color to venom.

Application

L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.

Biochem/physiol Actions

L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg2+ for its activation. The N-terminal region is essential for FAD binding. The H2O2 generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis. LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A9253-250MG:
A9253-25MG:
A9253-100MG-PW:
A9253-250MG-PW:
A9253-100MG:
A9253-BULK:
A9253-500MG:
A9253-VAR:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus)
Rokyta DR, et al.
BMC Genomics, 13(1), 312-312 (2012)
An enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process
Borges EP and Reis BF
Journal of the Brazilian Chemical Society, 16(6), 1226-1232 (2005)
Snake venom L-amino acid oxidases: trends in pharmacology and biochemistry
Izidoro LFM, et al.
BioMed Research International, 2014(15), 7546-7551 (2014)
Characterization of L-amino Acid Oxidase Derived from Crotalus adamanteus Venom: Procoagulant and Anticoagulant Activities
Nielsen VG
International Journal of Molecular Sciences, 20(19), 4853-4853 (2019)
Crystalline L-amino acid oxidase of Crotalus adamanteus
Wellner D and Meister A
The Journal of Biological Chemistry, 235(7), 2013-2018 (1960)

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