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A7674

Sigma-Aldrich

Anti-AOP1 antibody, Mouse monoclonal

clone AOP-38, purified from hybridoma cell culture

Synonym(s):

Anti-Antioxidant-like Protein 1, Anti-PRDX3, Anti-Peroxiredoxin 3

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About This Item

MDL number:
MFCD03096615
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AOP-38, monoclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa

species reactivity

monkey, chicken, canine, rat, human, mouse, hamster

concentration

~2 mg/mL

technique(s)

indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using a whole extract of cultured human acute T cell leukemia, Jurkat, cells

isotype

IgG1

UniProt accession no.

P30048

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PRDX3(10935)
mouse ... Prdx3(11757)
rat ... Prdx3(64371)

General description

Anti-AOP1 (antioxidant-like protein 1) antibody, mouse monoclonal, (antioxidantlike protein 1) (mouse IgG1 isotype) is derived from the AOP-38 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of human Aop1, conjugated to keyhole limpet hemocyanine (KLH). Aop1 (antioxidant-like protein 1, also called MER5, M.W. 25 kDa).
Antioxidant-like Protein 1 (AOP1) belongs to a protein family that may regulate oxidative stress defense.

Immunogen

synthetic peptide corresponding to C-terminal amino acids 236-256 of human Aop1, conjugated to KLH.

Application

AOP1 expression in cells isolated from human saliva was analyzed by western blot using mouse monoclonal anti-AOP1 antibody (A7674) at a 1:10000 dilution.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Mouse monoclonal anti-AOP1 antibody can be used for indirect ELISA and microarray applications.

Biochem/physiol Actions

AOP1 (antioxidant-like protein 1) is also proposed to be involved in the synthesis of glucosinolate. It defends oxidative stress and participates in proliferation and differentiation through antioxidant function and redox regulation. Monoclonal antibodies reacting specifically with Aop1 are useful tools for the determination of the functional activity of Aop1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A7674-.2ML:
A7674-BULK:
A7674-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Jäschke et al.
Journal of molecular biology, 277(4), 763-769 (1998-05-30)
Cyclophilins (CyPs) define a family of proteins binding to the immunosuppressive drug cyclosporin A (CsA). They are evolutionary highly conserved proteins being present in both pro- and eukaryotes and in different subcellular locations. CyPs possess enzymatic activity, namely peptidyl-prolyl cis-trans
Mammalian antioxidant protein complements alkylhydroperoxide reductase (ahpC) mutation in Escherichia coli
Tsuji K, et al.
The Biochemical Journal, 307(2), 377-381 (1995)
Jin-A Park et al.
Molecular medicine reports, 14(4), 3215-3219 (2016-08-12)
Antioxidant-like protein-1 (AOP-1) reduces the intracellular level of reactive oxygen species. In the present study, the age‑related change in AOP‑1 expression in the hippocampus among young, adult and aged gerbils was compared using western blot analysis and immunohistochemistry. The results demonstrated that
Transcript residency on ribosomes reveals a key role for the A rabidopsis thaliana bundle sheath in sulfur and glucosinolate metabolism
Aubry S, et al.
The Plant Journal, 78(4), 659-673 (2014)
Hongwei Xie et al.
Molecular & cellular proteomics : MCP, 7(3), 486-498 (2007-11-30)
Whole human saliva possesses tremendous potential in clinical diagnostics, particularly for conditions within the oral cavity such as oral cancer. Although many have studied the soluble fraction of whole saliva, few have taken advantage of the diagnostic potential of the
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