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A7550

Sigma-Aldrich

Achromopeptidase from Achromobacter lyticus

lyophilized powder, Protein ~5 % by biuret, 300-600 units/mg solid

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About This Item

CAS Number:
123175-82-6
Enzyme Commission number:
3.4.21.50 (BRENDA, IUBMB)
MDL number:
MFCD00130333
UNSPSC Code:
12352204

form

lyophilized powder

specific activity

300-600 units/mg solid

composition

Protein, ~5% biuret

foreign activity

Collagenase, present

storage temp.

−20°C

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.

Unit Definition

One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).

Physical form

Crude powder containing salts and medium components

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P342 + P311

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A7550-BULK:
A7550-VAR:
A7550-100KU:
A7550-1MU:
A7550-500KU:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Beatrice Quevedo et al.
BMC microbiology, 11, 14-14 (2011-01-21)
The purpose of this study was to design and evaluate fluorescent in situ hybridization (FISH) probes for the single-cell detection and enumeration of lactic acid bacteria, in particular organisms belonging to the major phylogenetic groups and species of oral lactobacilli
Niamh Toomey et al.
Applied and environmental microbiology, 75(10), 3146-3152 (2009-03-10)
Three wild-type dairy isolates of lactic acid bacteria (LAB) and one Lactococcus lactis control strain were analyzed for their ability to transfer antibiotic resistance determinants (plasmid or transposon located) to two LAB recipients using both in vitro methods and in
T Masaki et al.
Biochimica et biophysica acta, 660(1), 44-50 (1981-07-24)
Achromobacter lyticus M497-1 produces three kinds of alkaline proteases (protease I, II and III) in culture medium along with the bacteriolytic enzyme (Masaki, T., Nakamura, K., Isono, M. and Soejima, M. (1978) Agric. Biol. Chem. 42, 1443--1445). Among these three
T Masaki et al.
Biochimica et biophysica acta, 660(1), 51-55 (1981-07-24)
The unique specificity of Achromobacter protease I for lysine residue was investigated using synthetic and natural substrates, i.e., lysine derivatives, arginine derivatives, lysine vasopressin, substance P, ACTH and insulin. The enzyme cleaved only the -Lys-X- bonds in the above substrates.
Raju Sekar et al.
Applied and environmental microbiology, 69(5), 2928-2935 (2003-05-07)
We tested a previously described protocol for fluorescence in situ hybridization of marine bacterioplankton with horseradish peroxidase-labeled rRNA-targeted oligonucleotide probes and catalyzed reporter deposition (CARD-FISH) in plankton samples from different lakes. The fraction of Bacteria detected by CARD-FISH was significantly
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