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A4550

Sigma-Aldrich

Agar

Type A, suitable for plant cell culture

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
EC Number:
232-658-1
MDL number:
MFCD00081288
UNSPSC Code:
50405901
eCl@ss:
42040102
NACRES:
NA.21

biological source

algae (Rhodophyceae)

Quality Level

200

type

Type A

form

powder

technique(s)

cell culture | plant: suitable

transition temp

transition temp 32-38 °C (1.5%)

gel strength

>650 g/cm2

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar (Type A) is extracted from agarocytes of the red alga Rhodophyceae. It is a polysaccharide complex, consisting of a neutral gelling fraction, agarose, and a non-gelling fraction, agaropectin. Type-A agar forms a thermoreversible gel, and is most suitable for plant cell culture.

Application

Type A agar has been used:
  • to perform metal(loid)-related phenotyping of the Arabidopsis thaliana metal-sensitive mutant strains
  • as a component of Murashige and Skoog (MS) media to culturing Arabidopsis thaliana seeds
  • to culture Cicer arietinum L. (Chickpea) seeds for Agrobacterium rhizogenes mediated root transformation

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Journal of experimental botany, 71(22), 7301-7315 (2020-08-30)
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