A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

• Synonym(s): Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt
• Empirical Formula (Hill Notation): C₂₃H₃₅N₉O₄S · C₂H₄O₂
• CAS Number: 96087-38-6
• Molecular Weight: 593.70
• MDL number: MFCD00210085
• UNSPSC Code: 12352200
• PubChem Substance ID: 24890604
• NACRES: NA.52

Properties

• grade: Molecular Biology; for molecular biology
• Quality Level: 200
• Assay: ≥95%
• form: film or powder
• solubility: H₂O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
• storage temp.: −20°C
• SMILES string: CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
• InChI: 1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1
• InChI key: FFBLNTOMOSLSQM-AYEYRVMASA-N

Description

General description

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Application

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Biochem/physiol Actions

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Caution

Light sensitive.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves