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A0855

Sigma-Aldrich

Anti-Auxin antibody, Mouse monoclonal

clone 1E11-C11, purified from hybridoma cell culture

Synonym(s):

Anti-IAA, Anti-Indole-3-acetic acid

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About This Item

MDL number:
MFCD07785405
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

recombinant

expressed in mouse cell line

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1E11-C11, monoclonal

form

PBS solution

species reactivity

plant

technique(s)

dot blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA
western blot: suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Auxin is a plant hormone that regulates physiological growth and development. This hormone regulates cellular functions such as elongation, turgor, division, and differentiation. Indoleacetic acid (IAA) is recognized as the main auxin in several plants. IAA is synthesized from tryptophan (Trp) using Trp dependent pathways,. Mouse monoclonal anti-auxin antibody recognizes indoleacetic acid but not free unmethylated IAA.

Immunogen

indoleacetic acid (IAA) carboxyl linked to ovalbumin.

Application

Indole-3-acetic acid was identified in developing P.abies spruce plant parrafin embedded formalin-fixed embryos by immunohistochemistry with the monoclonal anti-indole-3-acetic acid antibody.
Mouse monoclonal anti-auxin antibody has been used for immunofluorescence studies. The antibody can also be used for ELISA, dot blot and western blot analyses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A0855-BULK:
A0855-200UL:
A0855-VAR:

Certificates of Analysis (COA)

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Xiaoyan Qiu et al.
Molecular human reproduction, 8(11), 998-1004 (2002-10-25)
Insulin-like growth factor binding protein-1 (IGFBP-1) and prolactin are recognized as crucial signals for the initiation and maintainance of decidualization. The purpose of the study was to investigate the effect of mifepristone on the expression of IGFBP-1, prolactin and progesterone
Inger Hakman et al.
Tree physiology, 29(4), 483-496 (2009-02-11)
Auxin and polar auxin transport have been implicated in controlling embryo patterning and development in angiosperms but less is known from the gymnosperms. The aims of this study were to determine at what stages of conifer embryo development auxin and
Manjul Singh et al.
Scientific reports, 7(1), 16101-16101 (2017-11-25)
Plants possess exuberant plasticity that facilitates its ability to adapt and survive under challenging environmental conditions. The developmental plasticity largely depends upon cellular elongation which is governed by a complex network of environmental and phytohormonal signals. Here, we report role
T Mori et al.
American journal of reproductive immunology (New York, N.Y. : 1989), 26(3), 97-103 (1991-10-01)
Expression of CD4-like molecule on vitelline membrane of murine eggs was demonstrated by indirect immunofluorescence (IIF) test and immunoprecipitation corresponding to the expression of major histocompatibility complex (MHC) class II molecule on murine sperm detected by immunoblotting. This molecule showed
Andrew W Woodward et al.
Annals of botany, 95(5), 707-735 (2005-03-08)
The phytohormone auxin is critical for plant growth and orchestrates many developmental processes. This review considers the complex array of mechanisms plants use to control auxin levels, the movement of auxin through the plant, the emerging view of auxin-signalling mechanisms
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