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Safety Information

R1137

Sigma-Aldrich

Hind III from Haemophilus influenzae

Restriction Enzyme

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About This Item

CAS Number:
81295-22-9
Enzyme Commission number:
3.1.21.4 (BRENDA, IUBMB)
MDL number:
MFCD00166570
UNSPSC Code:
12352204

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

≥10000 units/mL
10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-A/AGCTT-3′
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes

Application

HindIII, a restriction endonuclease, is used in molecular biology applications to cleave DNA at the recognition site 5′-A/AGCTT-3′ to generate DNA fragments with cohesive 5′-ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SB (B8781)
Comment: Hind III under suboptimal reaction conditions will cleave secondary recognition sites (star activity).

Physical form

Solution in 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 1 mM dithioerythritol, 250 mM NaCl, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C

incubation buffer

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Description
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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

R1137-VAR:
R1137-5KU:
R1137-BULK:
R1137-5VL:
R1137-1KU:

Certificates of Analysis (COA)

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In the presence of 100 mM Tris buffer (pH 7.5) and 1-10 mM Mg2+ EcoRI endonuclease cleaves DNA at a specific nucleotide sequence and in a characteristic way: -GAATTC-. But if Mg2+ is replaced by Mn2+, the specificity of the
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C Kessler et al.
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The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Cong Zhu et al.
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Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
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