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Safety Information

R0260

Sigma-Aldrich

BamH I from Bacillus amyloliquefaciens H

Restriction Enzyme

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About This Item

CAS Number:
81295-09-2
Enzyme Commission number:
3.1.21.4 (BRENDA, IUBMB)
MDL number:
MFCD00163008
UNSPSC Code:
12352204

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-G/GATCC-3′
Cutting results: a 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.

Application

BamHI is a DNA restriction endonuclease that is used in molecular biology applications to cleave DNA at the recognition sequence 5′-G/GATCC-3′ to generate 5′-cohesive termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SB (B8781).

Physical form

Solution in 10 mM Tris-HCl, pH 7.4 , 1 mM EDTA, 1mM dithioerythritol, 300 mM KCl, 0.01% Polydocanol (v/v), 50% glycerol (v/v), at 4°C

incubation buffer

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

R0260-1KU:
R0260-5KU:
R0260-BULK:
R0260-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J H Ellis et al.
Journal of immunology (Baltimore, Md. : 1950), 156(8), 2700-2709 (1996-04-15)
CD80 and CD86 are cell surface glycoproteins expressed on a variety of professional APCs. They have attracted much attention due to their function as potent costimulators of T lymphocyte function through their interaction with CD28 and possibly CTLA4. Because inhibitors
Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.
G A Wilson et al.
Journal of molecular biology, 97(1), 123-125 (1975-09-05)
P Manivasakam et al.
Nucleic acids research, 29(23), 4826-4833 (2001-12-01)
Mammalian cells repair DNA double-strand breaks by illegitimate end-joining or by homologous recombination. We investigated the effects of restriction enzymes on illegitimate and homologous DNA integration in mammalian cells. A plasmid containing the neo(R) expression cassette, which confers G418 resistance
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
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