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94875

Sigma-Aldrich

Atto 680

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

MDL number:
MFCD03456138
UNSPSC Code:
12352116
PubChem Substance ID:
93577787
NACRES:
NA.32

product line

BioReagent

Assay

≥90% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

InChI

1S/C27H31N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-15H,4-10,16H2,1-3H3,(H-,31,32,33,34,35)

InChI key

MHHMNDJIDRZZNT-UHFFFAOYSA-N

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General description

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Application

Atto 680 has been used as a dye to study the fluorescence quantum yields of bioanalytically relevant red dyes.
Atto fluorescent labels are designed for high sensitivity applications, including single-molecule detection. Atto labels have rigid structures that do not show any cis-trans isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 680 shows a molar extinction of 120,000 and QY of 30% in water (40% in ethanol). Decay time is 1.8 ns.

Other Notes

New red absorbing fluorescent dye as a biophysical probe for binding interactions; used as fluorescence tracer in spray diagnostics.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

94875-1MG-BULK-F:
94875-BULK-F:
94875-1MG-F:
94875-VAR-F:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD2363
BCBV6493
1343528
1338355
1331292

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Spray diagnostics using an all-solid-state Nd:YAIO3 laser and fluorescence tracers in commercial gasoline and diesel fuels.
I. Duwell et al.
Applied Physics B: Lasers and Optics, 79, 249-254 (2004)
Integrating sphere setup for the traceable measurement of absolute photoluminescence quantum yields in the near infrared
Wu?rth C, et al.
Analytical Chemistry, 84(3), 1345-1352 (2012)
Dual laser fluorescence correlation spectroscopy as a biophysical probe of binding interactions - evaluation of new red fluorescent dyes.
K.M. Swift et al.
Proc. SPIE: Int. Soc. Opt. Eng., 4252, 47-47 (2001)
Volker Buschmann et al.
Bioconjugate chemistry, 14(1), 195-204 (2003-01-16)
The spectroscopic characteristics (absorption, emission, and fluorescence lifetime) of 13 commercially available red-absorbing fluorescent dyes were studied under a variety of conditions. The dyes included in this study are Alexa647, ATTO655, ATTO680, Bodipy630/650, Cy5, Cy5.5, DiD, DY-630, DY-635, DY-640, DY-650
Philip Tinnefeld et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 6(2), 217-222 (2005-03-09)
Light-harvesting complexes, one of nature's supreme examples of nanoscale engineering, have inspired researchers to construct molecular optical devices, such as photonic wires, which are optimised for efficient transfer of excited-state energy over large distances. The control parameters for the design
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