Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

94280

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

powder, ~1 U/mg

Synonym(s):

Jack bean urease, Urea amidohydrolase

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9002-13-5
Enzyme Commission number:
3.5.1.5 (BRENDA, IUBMB)
EC Number:
232-656-0
MDL number:
MFCD00070858
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

form

powder

Quality Level

100

specific activity

~1 U/mg

mol wt

Mr ~480000

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease consists of nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.

Application

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.
Urease from Canavalia ensiformis (Jack bean) has been used in purification and crystallization studies.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25 °C.

Other Notes

Application in (selective) hydrolysis/condensations; structure.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

94280-BULK:
94280-25G:
94280-VAR:
94280-5G:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

K Takishima et al.
European journal of biochemistry, 175(1), 151-165 (1988-07-15)
The amino acid sequence of jack bean urease has been determined. The protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The subunit relative molecular mass calculated from the sequence is 90,770, indicating that urease
M J Sculley et al.
Biophysical chemistry, 19(1), 39-47 (1984-01-01)
The use of bifunctional reagents to form cross-links between subunits in protein oligomers and subsequent disruption of noncovalent interactions with SDS allows comment upon the number of subunits and the symmetry in the original assembly. In existing treatments the number
Isothermal titration calorimetry to characterize enzymatic reactions
Methods in Enzymology, 567, 215-236 (2016)
I.A. Natchev
Tetrahedron, 44, 1511-1511 (1988)
M.C.N. Jayasuriya et al.
Anim. Feed Sci. Technol., 8, 271-271 (1983)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service