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76245

Sigma-Aldrich

Atto 655 NHS ester

BioReagent, suitable for fluorescence

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About This Item

MDL number:
MFCD03456137
UNSPSC Code:
12352108
PubChem Substance ID:
93577785
NACRES:
NA.32

product line

BioReagent

Quality Level

100

Assay

≥90.0% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

extent of labeling

≥90.0%

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 650.0-656.0 nm Amax

suitability

suitable for coupling to amines
suitable for fluorescence

storage temp.

−20°C

SMILES string

[S](=O)(=O)([O-])CC1CC([N+](=C3C1=CC4=Nc5c(cc6c(c5)CCCN6CC)OC4=C3)CCCC(=O)ON2C(=O)CCC2=O)(C)C

InChI

1S/C31H36N4O8S/c1-4-33-11-5-7-19-13-22-26(15-24(19)33)42-27-16-25-21(14-23(27)32-22)20(18-44(39,40)41)17-31(2,3)34(25)12-6-8-30(38)43-35-28(36)9-10-29(35)37/h13-16,20H,4-12,17-18H2,1-3H3

InChI key

SPDKWNQUMMQOKX-UHFFFAOYSA-N

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Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

76245-BULK-F:
76245-50MG-F:
76245-0.25MG-KC:
76245-0.25MG-F:
76245-1MG-BULK-F:
76245-1MG-F:
76245-1VL-F:
76245-0.25MG-FREE:
76245-VAR-F:
76245-5X0.25MG-KC:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5539
BCCK0548
BCCJ0102
BCCH8476
BCCG9693

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Fluorescent-labeled DNA is generated through enzymatic incorporation of fluorophore-linked 2'-deoxyribonucleoside-5'-triphosphates (dNTPs) by DNA polymerases. We describe the synthesis of a variety of dye-labeled dNTPs. Amino-linker-modified 5'-triphosphates of all four naturally occurring nucleobases were used as precursors. Commercially available dyes were
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Nucleic acids research, 31(10), 2636-2646 (2003-05-09)
The modification of nucleic acids using nucleotides linked to detectable reporter or functional groups is an important experimental tool in modern molecular biology. This enhances DNA or RNA detection as well as expanding the catalytic repertoire of nucleic acids. Here
Highly sensitive protease assay using fluorescence quenching of peptide probes based on photoinduced electron transfer.
Nicole Marmé et al.
Angewandte Chemie (International ed. in English), 43(29), 3798-3801 (2004-07-20)
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Biomedical optics express, 10(3), 1257-1272 (2019-03-21)
Open-top light-sheet microscopy is a technique that can potentially enable rapid ex vivo inspection of large tissue surfaces and volumes. Here, we have optimized an open-top light-sheet (OTLS) microscope and image-processing workflow for the comprehensive examination of surgical margin surfaces
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Steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. Among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. Especially, the red-absorbing dyes ATTO 655
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