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50492

Sigma-Aldrich

Atto Rho101 NHS ester

BioReagent, suitable for fluorescence

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

Quality Level

100

Assay

90% (Coupling efficiency with benzylamine, HPLC)

form

solid

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
586 nm

fluorescence

λex 586 nm; λem 610 nm

λ

in ethanol (0.1% trifluoroacetic acid)

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto Rho101 NHS ester is a derivative of the well-known dye Rhodamine 101 for coupling to bio-molecules such as DNA, RNA or proteins. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO Rho101 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.

Storage Class Code

11 - Combustible Solids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

50492-1MG:
50492-5MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kakishi Uno et al.
Journal of the American Chemical Society, 133(34), 13558-13564 (2011-08-09)
Photoswitchable or photoactivatable fluorescent dyes are potentially applicable to ultrahigh density optical memory media as well as super-resolution fluorescence imaging when the dyes are highly fluorescent and have large absorption coefficients. Here, we report on highly fluorescent photochromic dyes, which
Li-lin Jiang et al.
Guang pu xue yu guang pu fen xi = Guang pu, 31(5), 1348-1351 (2011-08-02)
The absorption, fluorescence and time-resolved fluorescence spectra of Rhodamine 101 dye in both methanol and acidic methanol solutions were measured. The authors achieved the characteristic information of the absorption and fluorescence spectra, and obtained the S1 lifetimes. The authors assigned
Knut Rurack et al.
Analytical chemistry, 83(4), 1232-1242 (2011-01-22)
The determination of the fluorescence quantum yields (QY, Φ(f)) of a series of fluorescent dyes that span the absorption/excitation and emission ranges of 520-900 and 600-1000 nm is reported. The dyes encompass commercially available rhodamine 101 (Rh-101, Φ(f) = 0.913)
Christian Würth et al.
Analytical chemistry, 84(3), 1345-1352 (2012-01-17)
There is an increasing interest in chromophores absorbing and emitting in the near-infrared (NIR) spectral region, e.g., for applications as fluorescent reporters for optical imaging techniques and hence, in reliable methods for the characterization of their signal-relevant properties like the

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