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Safety Information

3446-005-01

Sigma-Aldrich

Cultrex 3-D Culture Matrix Laminin I

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About This Item

UNSPSC Code:
12352200

biological source

mouse (Engelbreth-Holm-Swarm (EHS) mouse tumor)

sterility

sterile

Assay

≥90% (SDS-PAGE)

concentration

6 mg/mL

technique(s)

cell culture | mammalian: suitable

solubility

water: miscible

shipped in

dry ice

storage temp.

−70°C

General description

3-D Culture Matrix™ Mouse Laminin I may be used as a gel on which to grow cells, or as a media additive alone or in concert with other basement membrane components, to study cellular growth and differentiation in three dimensions in vitro. To offer the most standardized Laminin I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of 6 mg/ml (by absorbance and extinction coefficient).

Physical form

Solution in Dulbecco′s Modified Eagle′s medium containing 10 μg/ml gentamycin sulfate and no phenol red.

Other Notes

Functional Assay:
3-D Culture: Laminin I promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
Cell Attachment: Tested for the ability to promote cell attachment and spreading of MG63 human osteosarcoma cells.
Sterility Testing:
No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test.No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
Storage Conditions and Product Stability:
Product is stable for a minimum of 3 months from date of shipment when stored at -80 °C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.

Legal Information

3-D Culture Matrix is a trademark of Trevigen, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

3446-005-01:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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