Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

14420C

SAFC

EX-CELL® 420 Serum-Free Medium for Insect Cells

with L-glutamine, sterile-filtered, suitable for insect cell culture

Pharma Manufacturing

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD01634638
UNSPSC Code:
12352207
NACRES:
NA.75

description

for research or for further manufacturing use

Quality Level

500

sterility

sterile-filtered

product line

EX-CELL®

form

liquid

technique(s)

cell culture | insect: suitable

components

HEPES: no
L-glutamine: yes
glucose: yes
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or serum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

14420-100B3056:
14420-20B1245:
14420-5B1172:
14420-1000M4180:
14420-73B3386:
14420-13B1212:
14420C-10B:
14420-20B3722:
14420-8.5BB1172:
14420-8BA1172:
14420-8BA4180:
14420-10Q4419:
14420-8B3386:
14420-8.5B1172:
14420-8B1172:
14420-1000M1675:
14420-20BA3175:
14420-10B1174:
14420-1000M:
14420-10B3056:
14420-1000M1172:
14420-10BD:
14420-6.2B1172:
14420-1B5839M:
14420-20BA1245:
14420C-1B:
14420-1000M1245:
14420-200B3056-CO:
14420-7B3606:
14420-20B3265:
14420C-1000ML:
14420-20B1172:
14420-64.5B3386:
14420-20B3647:
14420-500M:
14420-20B1675:
14420-1BACDA:
14420-1B5838M:
14420-8B3175:
14420-10BC:
14420-18B3175:
14420C-BULK:
14420-10B1252:
14420CPROC:
14420-100B3386:
14420-1000M1159:
14420-20B4419:
14420-7.11B3175:
14420-7B3722:
14420-4B3175:
14420-91B3386:
14420-80B3175:
14420C-PH:
14420-10B3647:
14420-10B1159:
14420-10B1172:
14420-10B3722:
14420-19B1212:
14420-18BA3175:
14420-1000M3056:
14420-1000M3722:
14420C-500ML:
14420-8.9B4180:
14420-1000M4313:
14420-1000M3659:
14420-100Q4419:
14420-8.5BA1172:
14420-50B1245:
14420-20Q4419:
14420-1000M3386:
14420-10Q3369:
14420-10BA4180:
14420C-10BC:
14420-44B3175:
14420-62B3386:
14420-100BA3386:
14420-20B1174:
14420-1000M1174:
14420-8BA3175:
14420-7BA3606:
14420-2000M1172:
14420-1000M3175:
14420-1BACD:
14420-1000M3265:
14420-50B1174:
14420-10B:
14420-40B1174:
14420-1B3056:
14420-20BA1172:
14420-10BA1252:
14420-4BA3175:
14420-10B3386:
14420-8B4180:
14420-20B3175:
14420-9.4B1172:
14420-1000M4419:
14420C-10BB:
14420-1B4419:
14420-200B3056:
14420-8B1174:
14420-10B4180:
14420-100B3722:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 2

1 of 2

Sigma-Aldrich

Sigma-Aldrich

T3285

TNM-FH insect medium

EX-CELL® CD Insect Cell Medium Chemically defined, sterile-filtered, suitable (for insect cell culture)

SAFC

14380C

EX-CELL® CD Insect Cell Medium

Hideki Yamaji et al.
Journal of bioscience and bioengineering, 95(2), 185-187 (2005-10-20)
When rat protein kinase C-delta (PKC-delta) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-delta content decreased in the late period while the extracellular PKC-6 markedly increased. During the
Norikatsu Nishikawa et al.
Cytotechnology, 43(1-3), 3-10 (2008-11-13)
The production of beta-galactosidase by Sf9 cells infected with recombinant Autographa californica nucleopolyhedrovirus (AcNPV) was investigated in shake-flask culture using two serum-free basal media: Grace's medium and TNM-FH (Grace's medium supplemented with lactalbumin hydrolysate and yeast extract). At the time
Fabiana R X Batista et al.
Cytotechnology, 49(1), 1-9 (2008-11-13)
Animal cells can be cultured both in basal media supplemented with fetal bovine serum (FBS) and in serum-free media. In this work, the supplementation of Grace's medium with a set of nutrients to reduce FBS requirements in Spodoptera frugiperda (Sf9)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service