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Safety Information

13110

Sigma-Aldrich

N-Benzoyl-L-tyrosine ethyl ester

≥98.0% (NT)

Synonym(s):

BTEE

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About This Item

Linear Formula:
4-(HO)C6H4CH2CH(NHCOC6H5)CO2C2H5
CAS Number:
3483-82-7
Molecular Weight:
313.35
Beilstein:
2223819
EC Number:
222-469-2
MDL number:
MFCD00002388
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
329750011

product name

N-Benzoyl-L-tyrosine ethyl ester, ≥98.0% (NT)

Assay

≥98.0% (NT)

form

powder or crystals

optical activity

[α]20/D −26±3°, c = 1% in ethanol

color

white

mp

118-121 °C (lit.)
118-122 °C

solubility

methanol: 1 g/10 mL, clear, colorless to almost colorless (















)

SMILES string

CCOC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)c2ccccc2

InChI

1S/C18H19NO4/c1-2-23-18(22)16(12-13-8-10-15(20)11-9-13)19-17(21)14-6-4-3-5-7-14/h3-11,16,20H,2,12H2,1H3,(H,19,21)/t16-/m0/s1

InChI key

SRLROPAFMUDDRC-INIZCTEOSA-N

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General description

N-Benzoyl-L-tyrosine ethyl ester (BTEE) is a substrate which is completely resistant to trypsin and is required for the assay of chymotrypsin.

Application

N-Benzoyl-L-tyrosine ethyl ester has been used for measuring chymotrypsin activity.

Other Notes

Substrate, completely resistant to trypsin, for the assay of chymotrypsin

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

13110-25G-F:
13110-5G-F:
13110-VAR-F:
13110-BULK-F:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K.A. Walsh et al.
Methods in Enzymology, 19, 31-31 (1979)
Substrate solubilization for the Hummel alpha-chymotrypsin assay.
K N Rao et al.
Analytical biochemistry, 65(1-2), 548-551 (1975-05-12)
I N Topchieva et al.
Bioconjugate chemistry, 11(1), 22-29 (2000-01-19)
A new method of preparation of noncovalent complexes between poly(ethylene glycol) (PEG) and proteins (alpha-chymotrypsin (ChT), lysozyme, bovine serum albumine) under high pressure has been developed. The involvement of polymer in the complexes was proved using (3)H-labeled PEG. The composition
K Matsumura et al.
Gamete research, 23(3), 255-266 (1989-07-01)
Probable participation of sperm protease in the acrosome reaction was investigated using several inhibitors and substrates. Among those examined, L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) and chymostatin, chymotrypsin inhibitors, p-nitrophenyl-p'-guanidinobenzoate (NPGB), a serine protease inhibitor, and N-benzoyl-L-tyrosine ethyl ester (BTEE), a chymotrypsin
A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin.
B C HUMMEL
Canadian journal of biochemistry and physiology, 37, 1393-1399 (1959-12-01)
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