Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

127-2.5

Sigma-Aldrich

Poly-D-Lysine & Laminin (2.5 ML)

Synonym(s):

Collagen IV from human placenta

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.75

form

liquid

Quality Level

100

shelf life

1 yr

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Poly-D-Lysine (PDL) and Laminin solution is ideal for use in the coating protocol for cell culture surfaces. PDL Laminin coating is extensively used for neural cultures. It is known to enhance cell adhesion and cell maturation during in vitro cell culture. Poly-D-Lysine and Laminin solution is prepared in phosphate-buffered saline and sterile filtered. It is used to coat tissue culture ware to culture surface ratio of 0.25 ml/cm2. Tissue culture ware is coated at room temperature for one hour and carefully aspirate the solution. After wash two times with PBS, coated tissue culture ware may be used immediately or air-dried and stored at 4oC for up to one week.

Application

Poly-D-Lysine & Laminin (2.5 ML) has been used in vitro as inductive agents to stimulate Y79 cells to express both neuronal and glial characteristics.It has been used to coat culture plates for retinal ganglion cell culture.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

127-2.5:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

J Tombran-Tink et al.
Investigative ophthalmology & visual science, 30(8), 1700-1707 (1989-08-01)
Tumor cells can be induced to differentiate in vitro by biochemical manipulation of their culture environment. In the studies described here, the effects of medium conditioned by human retinal pigmented epithelial (RPE) cells on Y79 human retinoblastoma cells have been
Jing-Wen Yu et al.
Journal of molecular neuroscience : MN, 60(4), 486-497 (2016-08-31)
Bone marrow-derived mesenchymal stem cells (MSCs) are the ideal transplanted cells of cellular therapy for promoting neuroprotection and neurorestoration. However, the optimization of transplanted cells and the improvement of microenvironment around implanted cells are still two critical challenges for enhancing

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service