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11012001

FLO-1 cell line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Synonym(s):

FLO1 Cells

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About This Item

UNSPSC Code:
41106514

biological source

human esophagus

packaging

tube of 5 μg 11012001-DNA-5UG
pkg of vial of cells 11012001-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

P53 mutation c.830G>T

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

Related Categories

Cell Line Origin

Distal oesophageal adenocarcinoma

Application

For complete product information, please see ECACC

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 12,14
D7S820: 8
THO1: 6
TPOX: 9,11
vWA: 16

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 105 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

11012001-1VL:

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Certificates of Analysis (COA)

Lot/Batch Number

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Mathieu Francois Derouet et al.
PloS one, 9(12), e115589-e115589 (2015-01-01)
Carcinoma of the esophagus has a high case fatality ratio and is now the 6th most common cause of cancer deaths in the world. We previously conducted a study to profile the expression of miRNAs in esophageal adenocarcinoma (EAC) pre
S J Hughes et al.
Cancer research, 57(24), 5571-5578 (1998-01-04)
This study describes Fas (CD95) expression in Barrett's esophagus, adenocarcinomas of the esophagus, and three esophageal adenocarcinoma cell lines. Immunohistochemical analysis of Barrett's esophagus demonstrated cell surface expression of Fas protein. In contrast, 30.5% of esophageal adenocarcinomas examined by immunohistochemical
Sarah Derks et al.
Cancer immunology research, 3(10), 1123-1129 (2015-06-18)
Esophageal adenocarcinoma is an increasingly common disease with a dismal 5-year survival rate of 10% to 15%. In the first systematic evaluation of the PD-1 pathway in esophageal adenocarcinoma, we identify expression of PD-L2 in cancer cells in 51.7% of
Jurjen J Boonstra et al.
Journal of the National Cancer Institute, 102(4), 271-274 (2010-01-16)
For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the

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