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Safety Information

07679

Sigma-Aldrich

Abberior® STAR 635P, NHS ester

suitable for fluorescence

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

mol wt

Mw 1030 g/mol

solubility

DMF: 0.25 mg/mL, clear

fluorescence

λex 634 nm; λem 654 nm±10 nm in PBS, pH 7.4

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Abberior STAR635P is the first member of an entirely new class of dyes introduced exclusively by Abberior - first commercially available phosphorylated fluorescent dye. The dye excels in its photophysical parameters, e.g. very bright, very stable and it enables basically background free imaging.
Abberior STAR 635P can substitute Atto™ 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength of 750 - 780 nm is recommended. Please see reference1 for detailled characteristics.
Best results are obtained with freshly prepared samples.

Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED.

Key Features
  • First phosphorylated dye
  • Unmatched, background free STED imaging contrast
  • Verified in the Leica STED system

Absorption Maximum, λmax: 638 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 120′000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.21 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.40 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 651 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 - 780 nm
Fluorescence Quantum Yield, η: 0.90 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.3 ns (PBS, pH 7.4)

Suitability

Designed and tested for fluorescent super-resolution microscopy

Other Notes

Find more infomation here Abberior®

Legal Information

Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

07679-1MG:
07679-1MG-BULK:
07679-BULK:

Certificates of Analysis (COA)

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Sören Brandenburg et al.
Frontiers in physiology, 9, 1227-1227 (2018-10-24)
Rationale: Recently, abundant axial tubule (AT) membrane structures were identified deep inside atrial myocytes (AMs). Upon excitation, ATs rapidly activate intracellular Ca2+ release and sarcomeric contraction through extensive AT junctions, a cell-specific atrial mechanism. While AT junctions with the sarcoplasmic

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