Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

05482

Sigma-Aldrich

Bovine Serum Albumin

fraction V, ≥96% (GE)

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

Assay

≥96% (GE)

form

lyophilized powder

mol wt

~66 kDa
Mr ~67000

purified by

heat shock fractionation

loss

≤5% loss on drying

pH

5.0-6.0 (10 mg/mL in H2O)

solubility

H2O: 40 mg/mL, clear to faintly turbid, faintly yellow to greenish-yellow
H2O: slightly soluble

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Application

Albumin from bovine serum has been used as a test compound for detecting the weaker, distal chromatin co-associations by using enhanced 4C (e4C, enhanced chromosome conformation capture on chip) technique.

Biochem/physiol Actions

Bovine serum albumin also referred to as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated as well as non-treated microwells. It can also trigger insulin dependent diabetes mellitus in humans.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

By a modified Cohn procedure, using cold ethanol, pH and low temperature precipitation.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Other Notes

As chiral complexing agent in the resolution of dansyl DL amino acids
Sales restrictions may apply

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05482-VAR:
05482-100G:
05482-25G:
05482-500G:
05482-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Bovine Serum Albumin heat shock fraction, pH 7, ≥98%

Sigma-Aldrich

A9647

Bovine Serum Albumin

Tom Sexton et al.
Nature protocols, 7(7), 1335-1350 (2012-06-23)
Chromosome conformation capture (3C) is a powerful technique for analyzing spatial chromatin organization in vivo. Technical variants of the assay ('4C') allow the systematic detection of genome-wide coassociations with bait sequences of interest, enabling the nuclear environments of specific genes
L. Lepri et al.
Chromatographia, 36, 297-297 (1993)
J Karjalainen et al.
The New England journal of medicine, 327(5), 302-307 (1992-07-30)
Cow's milk has been implicated as a possible trigger of the autoimmune response that destroys pancreatic beta cells in genetically susceptible hosts, thus causing diabetes mellitus. Studies in animals have suggested that bovine serum albumin (BSA) is the milk protein
M Steinitz
Analytical biochemistry, 282(2), 232-238 (2000-06-30)
ELISA provides a highly sensitive procedure for quantitating antigens and antibodies. In that assay, microwells are coated initially with a specific ligand and then saturated with inert molecules to minimize nonspecific background. Coating can be improved by pretreating the microwells
Preparation and properties of serum and plasma proteins; a system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids.
E J COHN et al.
Journal of the American Chemical Society, 68, 459-475 (1946-03-01)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service