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03004

Sigma-Aldrich

Abberior® STAR 512, maleimide

for STED application

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About This Item

UNSPSC Code:
12352111
NACRES:
NA.32

mol wt

Mw 861.7 g/mol

concentration

≥50% (degree of coupling)

solubility

DMF: 0.25 mg/mL, clear

fluorescence

λex 512 nm; λem 530 nm±5 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Absorption Maximum, λmax: 517 nm (MeOH),
511 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.24 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.07 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 533 nm (MeOH),
530 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 −620 nm
Fluorescence Quantum Yield, η: 0.82 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 4.1 ns (PBS, pH 7.4)

Application

Abberior® Star 512 labelled phosphoethanolamine lipid analogues were used for gated STED-FCS (stimulated emission depletion - fluorescence correlation spectroscopy) study.

Suitability

Designed and tested for fluorescent super-resolution microscopy

Other Notes

Find more infomation here Abberior®

Legal Information

abberior is a registered trademark of Abberior GmbH

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

03004-1MG-BULK:
03004-1MG:

Certificates of Analysis (COA)

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Mathias P Clausen et al.
Methods (San Diego, Calif.), 88, 67-75 (2015-07-01)
Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these approaches is the combination of far-field super-resolution stimulated-emission-depletion (STED) microscopy with
S W Hell et al.
Optics letters, 19(11), 780-782 (1994-06-01)
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported
Stefan W Hell
Nature biotechnology, 21(11), 1347-1355 (2003-11-05)
For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to
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