Polyvinylpyrrolidone (PVP) can form complexes with phenolics and alkaloids. The complex formation allows removal of phenolics and alkaloids from plant samples, thereby inhibiting any modification of proteins by them and any hindrance they may cause in spectrophotometric determinations of protein content. PVP might also enhances the stability of enzymes.
Polyvinylpyrrolidone has been used in the purification of glutamine synthetases from plant samples. It has been used to block the PVDF (polyvinylidene difluoride) membrane and to avoid nonspecific binding.
50, 100, 500 g in poly bottle
1 kg in poly bottle
5 kg in poly drum
Polyvinylpyrrolidone is a component of Denhardt′s Solution and is included at a concentration of 1% (w/v) in the standard 50X stock solution.