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Thiamine hydrochloride

Pharmaceutical Secondary Standard; Certified Reference Material

Aneurine hydrochloride, Vitamin B1 hydrochloride
Empirical Formula (Hill Notation):
C12H17ClN4OS · HCl
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


certified reference material
pharmaceutical secondary standard




current certificate can be downloaded


HPLC: suitable
gas chromatography (GC): suitable


250 °C (dec.) (lit.)

Featured Industry

Food and Beverages



pharmacopeia traceability

traceable to PhEur Y0000467
traceable to USP 1656002

SMILES string




InChI key


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General description

Certified pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to pharmacopeia primary standards
Thiamine hydrochloride belongs to the class of water soluble, B vitamins. It is present most commonly in food-stuffs either in free form or phosphorylated form.


These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.
Thiamine hydrochloride may be used as a standard in the determination of thiamine hydrochloride in food samples using high performance liquid chromatographic system coupled with fluorescence detector (HPLC/fluorimetry). It may be used pharmaceutical reference standard for the quantification of the analyte in pharmaceutical formulations using high-performance liquid chromatography technique.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.


To see an example of a Certificate of Analysis for this material enter LRAB9939 in the slot below. This is an example certificate only and may not be the lot that you receive.


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Signal Word


Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Extraction procedures for the liquid chromatographic determination of thiamin, riboflavin and vitamin B6 in foodstuffs
Ndaw.S, et al.
Food Chemistry, 71, 129-138 (2000)
Reversed-phase high-performance liquid chromatography method with coulometric electrochemical and ultraviolet detection for the quantification of vitamins B1 (thiamine), B6 (pyridoxamine, pyridoxal and pyridoxine) and B12 in animal and plant foods
Lebiedzinska A, et al.
Journal of Chromatography A, 1173, 71-80 (2007)
High-performance liquid chromatography method for the simultaneous determination of thiamine hydrochloride, pyridoxine hydrochloride and cyanocobalamin in pharmaceutical formulations using coulometric electrochemical and ultraviolet detection
Marszall LM, et al.
Journal of Chromatography A, 1094(1-2), 91-98 (2005)
Joseph E Kerschner et al.
Archives of otolaryngology--head & neck surgery, 135(1), 33-39 (2009-01-21)
To investigate genetic differences in middle ear mucosa (MEM) with nontypeable Haemophilus influenzae (NTHi) infection. Genetic upregulation and downregulation occurs in MEM during otitis media (OM) pathogenesis. A comprehensive assessment of these genetic differences using the techniques of complementary DNA...
Ashish K Singh et al.
Alcoholism, clinical and experimental research, 38(7), 2127-2137 (2014-06-17)
Chronic ethanol (EtOH) consumption is associated with oxidative tissue damage, decrease in antioxidant enzyme activities, and increase in hepatic and plasma lipids. This study investigates the effect of modified probiotic Escherichia coli Nissle 1917 (EcN) secreting pyrroloquinoline quinone (PQQ) against...


HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express C18

Pyridoxine hydrochloride, ≥98% (HPLC); Nicotinamide, ≥98.5% (HPLC); Thiamine hydrochloride, reagent grade, ≥99% (HPLC); Biotin, ≥99.0% (T); Folic acid, analytical standard; Cyanocobalamin (B12), analytical standard; Riboflavin (B2), analytical standard


LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5

Separation of Pyridoxine Hydrochloride (B6), analytical standard; Thiamine Hydrochloride (B1), analytical standard

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