N5514
Nitro Blue Tetrazolium
tablet
Synonym(s):
Nitrotetrazolium Blue Chloride
About This Item
Recommended Products
form
tablet
Quality Level
color
yellow
pH
4.5-5.5
solubility
soluble, clear, yellow
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI
1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key
FSVCQIDHPKZJSO-UHFFFAOYSA-L
General description
Application
- to measure superoxide radicals
- in lactate dehydrogenase quantification assay
- for blot development in western blotting analysis
- in NADPH-d histochemistry
- as a reagent to analyse the properties of murine intestinal mucins by electrophoresis and histology
- in immunohistochemistry
Quantity
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
FSL
Group 5: Self-reactive substances
Nitro compounds
Hazardous rank II
2nd self-reactive materials
ISHL Indicated Name
Substances Subject to be Indicated Names
ISHL Notified Names
Substances Subject to be Notified Names
JAN Code
N5514-25TAB:4548174007471
N5514-VAR:
N5514-10TAB:4548174007464
N5514-BULK:
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Articles
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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