CRM12900L

Escherichia coli O157 (NT) NCTC 12900 LENTICULE^® discs

30-120 CFU, suitable for microbiology, certified reference material, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

• Synonym(s): Microbiological standards, Certified reference material, E. coli O157 (NT) NCTC 12900 Lenticule^® discs
• UNSPSC Code: 41116107
• NACRES: NA.24

Properties

• grade: certified reference material
• Quality Level: 300
• Agency: ISO 17034; ISO/IEC 17025
• form: disc (hydrosoluble); solid
• shelf life: limited shelf life, expiry date on the label
• packaging: package of 10 discs
• manufacturer/tradename: Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
• application(s): environmental; food and beverages; microbiology
• storage temp.: −20°C
• suitability: Escherichia coli; coliforms

Description

General description

Escherichia coli O157 (NT) NCTC 12900 Lenticule^® discs are Certified Microorganisms Standards (Certified Microbiological Reference Materials), which can be used as quantitative and qualitative control organisms. They contain colony-forming units (CFUs) in the range of 30-120 per disc.

The technology allows long-term conservation and renders the organisms resistant to temperature changes. They are intended to be used as daily internal quality control organisms.

These microorganisms are certified according to ISO/IEC 17025 and produced under reproducible conditions compliant with ISO 17034 using authenticated strains from NCTC. These ready-to-use microbiological controls minimize the need for maintaining control strains in the test laboratory and guarantee the use of an authenticated control culture for every quality control test.

Lenticule^® discs are prepared from a traceable culture obtained freeze-dried from the National Collection of Type Cultures (NCTC) or National Collection of Pathogenic Fungi (NCPF) and are manufactured by Sigma-Aldrich under license from UK Health Security Agency.

Application

Escherichia coli O157 (NT) NCTC 12900 Lenticule^® discs are used as reference strains in routine quality control for food, beverage, water, and environmental testing. Additionally, they are excellent for use in method development and validations, as starter cultures, and for use in research studies and educational purposes.

Features and Benefits

• Ready-to-use at convenient CFU ranges
• No dilution required
• Quick rehydration in 10 minutes
• At least 16 months of shelf life in a standard -20 °C freezer
• Complete with a comprehensive certificate of analysis
• No maintenance of stock cultures
• No recovery time or pre-enrichment step is necessary
• Robust, efficient, and reliable
• Easy-to-use
• Cost-effective

Other Notes

Strain origin: NCTC 12900

Other cell culture collections:

• DSM 17076
• ATCC^® 700728
• CECT 4972

Click here to access BacDive database for more info on metabolism, genome, growth media and more

Strain History - Accessioned into the NCTC in 1996 < Dr Henry R. Smith (CPHL, Public Health Laboratory Service, Colindale, London, UK) < Isolated by Federal Public Health Lab (Innsbruck, Austria), from unknown substrate, Innsbruck Austria, in 1992

Legal Information

ATCC is a registered trademark of American Type Culture Collection

Lenticule is a registered trademark of The Secretary of State for Health (United Kingdom)

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3