C9157

Activated charcoal

suitable for cell culture, suitable for plant cell culture

• Synonym(s): Charcoal activated
• Empirical Formula (Hill Notation): C
• CAS Number: 7440-44-0
• Molecular Weight: 12.01
• EC Number: 231-153-3
• MDL number: MFCD00133992
• UNSPSC Code: 11101522
• PubChem Substance ID: 24893138
• NACRES: NA.21

Properties

• vapor pressure: <0.1 mmHg ( 20 °C)
• Quality Level: 200
• description: acid-washed with hydrochloric acid
• form: powder
• autoignition temp.: 842 °F
• resistivity: 1375 μΩ-cm, 20°C (graphite)
• technique(s): cell culture | mammalian: suitable; cell culture | plant: suitable
• mp: 3550 °C (lit.)
• SMILES string: [C]
• InChI: 1S/C
• InChI key: OKTJSMMVPCPJKN-UHFFFAOYSA-N

Description

General description

Activated charcoal is originally used as an absorbent to remove toxic substances from plants. The use of activated charcoal in culture media gives it a dark colour. It induces absorption of phenolic, cell lysis products, ethylene, methane and many other compounds that are known to be deleterious to the plant tissue. Along with this, activated charcoal also absorbs growth regulators and other media components. In orchid, carrot and tomato tissue culture, activated charcoal induces growth and differentiation.

Application

Activated charcoal has been used:

• in the pre-treatment of fetal calf serum, which was then used for protein content determination
• to study its effect on cell viability
• in a tissue culture medium and to study the availability of 2,4-dichlorophenoxyacetic acid in the same

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable