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B4252

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

chromogenic, ≥98%, powder

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
7240-90-6
Molecular Weight:
408.63
Beilstein:
1402009
EC Number:
230-640-8
MDL number:
MFCD00005666
UNSPSC Code:
12352204
PubChem Substance ID:
24891761
NACRES:
NA.83

product name

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%, powder

Quality Level

200

Assay

≥98%

form

powder

solubility

DMF: methanol (1:1): 50 mg/mL

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

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Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

B4252-50MG:4548173189024
B4252-500MG:4548173189017
B4252-100MG:4548173188966
B4252-VAR:
B4252-1G:4548173188980
B4252-2G:4548173189000
B4252-250MG:4548173188997
B4252-150MG:4548173188973
B4252-5MG:
B4252-150MG-KC:
B4252-BULK:
B4252-5G:4548173189031
B4252-10MG:

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Certificates of Analysis (COA)

Lot/Batch Number
0000289226
0000289226
SLCM6879
SLCM0868
SLCK5525

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