919233

Sodium 2-(4-hexylphenyl)diazene-1-sulfonate

(0.5% solution in 25mM aqueous ammonium bicarbonate, pH 7.0), ≥95%

• Synonym(s): 4-Hexylphenylazosulfonate, Azo
• Empirical Formula (Hill Notation): C₁₂H₁₇N₂O₃S · Na
• CAS Number: 158896-12-9
• Molecular Weight: 292.33
• UNSPSC Code: 12161503
• NACRES: NA.22

Properties

• Quality Level: 100
• Assay: ≥95%
• form: liquid
• refractive index: n/D 1.334
• density: 0.994 g/mL
• storage temp.: 2-8°C

Description

Application

4-Hexylphenylazosulfonate (Azo) is a photocleavable anionic surfactant developed by the Ge lab for use in high-throughput top-down and bottom-up proteomics by mass spectrometry (MS). Initially, the reagent showed a breakthrough as an improved method for top-down analysis [Brown et al. 2019]. Top-down analysis looks at intact proteins, while bottom-up analysis uses digested proteins. Of the two, top-down analysis is preferable for detecting post-translational modifications and sequence variations. Bottom-up is more commonly used and is likely to detect more proteins. This reagent was also shown to work for sample extraction that can be used for both top-down and bottom-up analysis on the same sample to maximize data collection[Brown et al. 2020].

Surfactants are required for protein extraction especially for challenging classes of protein including membrane proteins[Brown et al. 2020] and extracellular matrix proteins[Knott et al. 2020], but surfactants suppress the signal in MS. While removing a surfactant will improve the signal, this can cause protein loss and degradation. Azo allows for easy removal since it can be rapidly degraded using ultraviolet irradiation (λ_max = 305 nm). Using Azo in this way reduces protein loss and gives an improved signal. Please see the Ge lab publications for information on using this product [Brown et al. 2019, Brown et al. 2020, Knott et al. 2020, and Aballo et al. 2021].

Product can be used with our line of photoreactors: Including Penn PhD (Z744035) & SynLED 2.0 (Z744080)

Legal Information

PCT/US2019/035447

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2