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Key Documents

Safety Information

70184

Millipore

Antibiotic Broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Antibiotic Medium No. 3, Assay Broth, Penassay Broth, Grove and Randall Antibiotic Broth

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About This Item

UNSPSC Code:
41171504
NACRES:
NA.74

sterility

non-sterile

Quality Level

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dextrose, 1.0 g/L
dipotassium hydrogen phosphate, 3.68 g/L
meat extract, 1.5 g/L
peptone (from meat), 5.0 g/L
potassium dihydrogen phosphate, 1.32 g/L
sodium chloride, 3.5 g/L
yeast extract, 1.5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
food and beverages
life science and biopharma
pharmaceutical

microbiology

suitability

bacteria
nonselective for Escherichia coli
nonselective for Klebsiella spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for coliforms

Application

Used in antibiotic assay work (USP, FDA, Ph Eur) according to Grove & Randall; used in the turbidimetric assay of e.g. penicillin, bacitracin and kanamycin with S. aureus and for the cylinder assay of e.g. erythromycin and chloramphenicol with M. inteus.

Preparation Note

Dissolve 17.5 g in 1 litre of warm (60 C) distilled water and sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

70184-VAR:
70184-BULK:
70184-500G:
70184-100G:


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D.C. Grove et al.
Assay Methods of Antibiotics, Med. Encycl. (1955)

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