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60634

Sigma-Aldrich

Catalase from Micrococcus lysodeikticus

solution, dark brown

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About This Item

CAS Number:
9001-05-2
MDL number:
MFCD00081483
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Micrococcus lysodeikticus)

Quality Level

200

form

solution

specific activity

65,000-150,000 U/mL

mol wt

Mr ~230000

technique(s)

microbe id | metabolite detection: suitable

color

dark brown

solubility

0.05 M phosphate buffer pH 7.0: soluble, clear, brown (1:10)

Protein ID accession no.

UTX35086

UniProt accession no.

P29422

shipped in

wet ice

storage temp.

2-8°C

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

InChI key

NUVBSKCKDOMJSU-UHFFFAOYSA-N

General description

Research area: Cell Signaling

Catalase (CAT) is an antioxidant enzyme, encoded by the CAT gene family. The enzyme is ubiquitously expressed in most of the aerobic organisms.

Application

Catalase from Micrococcus lysodeikticus has been used to eliminate excess of hydrogen peroxide production during sugar analog synthesis by in vitro biocatalytic cascade. It has also been used as a standard to evaluate oxidative stress by indirectly measuring catalytic activity.

Biochem/physiol Actions

Catalase rapidly converts hydrogen peroxide to oxygen and water. It exhibits antioxidant activity and elicits a defense mechanism against reactive oxygen species (ROS). Catalase plays a role in neutralizing noxious hydrogen peroxide in nature as well as in various industries such as textile, pharma, and dairy. Micrococcus lysodeikticus catalase is a heme-containing protein.Catalase, along with various key cellular systems involved in hydrogen peroxide (H2O2) processing, plays a key role in regulating cellular redox balance and aids in cellular and intercellular signaling.

Unit Definition

1 U corresponds to the amount of enzyme which decomposes 1 μmol H2O2 per minute at pH 7.0 and 25 °C

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

60634-100ML:4548173376578
60634-VAR:
60634-BULK:
60634-500ML:4548173376585

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK4677
BCCJ3785
BCCD7800
BCCC7739
BCCB1588

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Hessam Sepasi Tehrani et al.
Progress in biophysics and molecular biology, 140, 5-12 (2018-03-14)
Catalase is one of the firsts in every realm of biological sciences. At the same time it also has a number of unusual features. It has one of the highest turnover numbers of all enzymes. It is essential for neutralizing
Pablo Burraco et al.
BMC evolutionary biology, 17(1), 164-164 (2017-07-08)
Adaptive plasticity is essential for many species to cope with environmental heterogeneity. In particular, developmental plasticity allows organisms with complex life cycles to adaptively adjust the timing of ontogenetic switch points. Size at and time to metamorphosis are reliable fitness
Ivan Gomez-Mestre et al.
PloS one, 8(12), e84266-e84266 (2013-12-21)
Many amphibian species exploit temporary or even ephemeral aquatic habitats for reproduction by maximising larval growth under benign conditions but accelerating development to rapidly undergo metamorphosis when at risk of desiccation from pond drying. Here we determine mechanisms enabling developmental
Margarita Florencio et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 242, 110654-110654 (2020-01-12)
Organisms are exposed to multiple environmental factors simultaneously to which they often respond behaviorally, morphologically and/or physiologically. Amphibian larvae are quite plastic and efficiently adjust their phenotype and physiology to the reigning local conditions. Here we tested whether the combination
An improved procedure using ferricyanide for detecting catalase isozymes.
W Woodbury et al.
Analytical biochemistry, 44(1), 301-305 (1971-11-01)
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