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318744

Sigma-Aldrich

Bromophenol Blue solution

0.04 wt. % in H2O

Synonym(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue sodium salt

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About This Item

Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
34725-61-6
Molecular Weight:
691.94
MDL number:
MFCD00013793
UNSPSC Code:
12171500
PubChem Substance ID:
24859143
NACRES:
NA.47

form

liquid

Quality Level

200

concentration

0.04 wt. % in H2O

technique(s)

titration: suitable

pH

3.0-4.6, yellow to blue

density

0.985 g/mL at 25 °C

λmax

589 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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General description

Bromophenol Blue is a tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

318744-100ML:
318744-BULK:
318744-6X100ML:
318744-VAR:
318744-500ML:

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Certificates of Analysis (COA)

Lot/Batch Number
MKCM9039
MKCK7679
MKCD9662
MKBX0023V
MKBR3561V

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Rapid transfer of DNA from agarose gels to nylon membranes.
Reed KC and Mann DA.
Nucleic Acids Research, 13, 7207-7221 (1985)
Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique.
Dzandu JK, et.al.
Proceedings of the National Academy of Sciences of the USA, 81, 1733-1737 (1984)
Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique.
Dzandu JK, et al.
Proceedings of the National Academy of Sciences of the USA, 81, 1733-1737 (1984)

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