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230251

Sigma-Aldrich

Eosin Y

75% (HPLC)

Synonym(s):

2′,4′,5′,7′-Tetrabromofluorescein, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, Eosin yellowish, Solvent red 43

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About This Item

Empirical Formula (Hill Notation):
C20H8Br4O5
CAS Number:
15086-94-9
Molecular Weight:
647.89
Colour Index Number:
45380:2
EC Number:
239-138-3
MDL number:
MFCD00036189
UNSPSC Code:
12171500
PubChem Substance ID:
24853789
NACRES:
NA.47

Quality Level

100

Assay

75% (HPLC)

form

powder

color

orange to very dark orange

solubility

spirit: soluble

λmax

524 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H8Br4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

InChI key

DBZJJPROPLPMSN-UHFFFAOYSA-N

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General description

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Application

Eosin Y has been used as a reagent in the preparation of Nigrosin-Eosin (NE) stain and is used in hematoxylin and eosin (H&E) staining technique to visualize alginate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

230251-100G:
230251-25G:
230251-BULK:
230251-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
MKCK7973
MKCD3439
MKBW2284V
MKBR2865V
MKBK3177V

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Electrophoretic detection of alginate by Hematoxylin and Eosin fluorescence: Implications in cell encapsulation/tissue engineering
Verma Y K
Reactive functional Polymers, 102, 130-136 (2016)
Concordance among pathologists in the second Cardiac Allograft Rejection Gene Expression Observational Study (CARGO II).
Crespo-Leiro MG, Zuckermann A, Bara C, et al.
Transplantation, 94(11), 1172-1177 (2012)
Gregorios J B
Histophatologic Techniques (2006)
Tawar Qaradakhi et al.
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Low circulating levels of undercarboxylated osteocalcin (ucOC) is associated with a higher risk of cardiovascular disease, yet whether ucOC has a direct effect on endothelium-dependent vasorelaxation, or in proximity to its postulated receptor, the class CG protein-coupled receptor (GPCR6A), in
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