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Safety Information

07689

Millipore

O′Meara′s Reagent

suitable for microbiology

Synonym(s):

Voges-Proskauer test with O’Meara Reagent

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About This Item

UNSPSC Code:
41171621
NACRES:
NA.85

product line

BioChemika

Quality Level

100

shelf life

limited shelf life, expiry date on the label

composition

creatine, 0.3 g
dist. water, 100 mL
potassium hydroxide, 40 g

packaging

pkg of 100 mL

storage condition

(Tightly closed. Dry.)

technique(s)

microbe id | metabolite detection: suitable

application(s)

environmental
food and beverages
microbiology

suitability

Enterobacter spp.
Klebsiella spp.
Neisseria spp.
Pseudomonas spp.

General description

O′Meara′s Reagent is used in the Voges-Proskauer (VP) test for the detection of acetoin production by bacterial strains. VP test is helpful in identifying members of the family Enterobacteriaceae based on their ability to produce acetylmethylcarbinol (AMC = acetonin) from glucose. Acetoin is an intermediate which is oxidized by oxygen under alkaline conditions (KOH) to 2,3-Butanediol. With creatinine (break down product from creatine) and the enhancement of α-naphtol a red color is formed (Meisenheimer complex)

Application

The O′Meara reagent is a component of the Voges-Proskauer (VP) test, which is a commonly used laboratory technique in industrial microbiology to differentiate between bacterial strains based on their ability to produce acetylmethylcarbinol (AMC) from glucose. The VP test is often used as a tool to identify and authenticate bacterial strains in the food, beverage, and pharmaceutical industries.

Analysis Note

To perform the VP test, a bacterial sample is inoculated into a nutrient broth and incubated. The O′Meara reagent, which contains a mixture of creatinine and potassium hydroxide (KOH), is then added to the culture. Acetoin produced in the medium by bacterial culture is oxidized under alkaline conditions in the presence of air to form diacetyl which reacts with creatine to give a pinkish-red compound.

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H290,H302,H314

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

07689-BULK-F:
07689-100ML-F:
07689-VAR-F:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG5985
BCCC8944
BCCC3670
BCBW8149
BCBV6702

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