ELONG-RO

Expand^™ Long Template PCR System

suitable for PCR, optimum reaction temp. 68 °C

• Synonym(s): long range pcr, long template pcr
• Enzyme Commission number: 2.7.7.7 (BRENDA, IUBMB)

Properties

• usage: sufficient for ≤190 reactions (11681842001); sufficient for ≤38 reactions (11681834001); sufficient for ≤950 reactions (11759060001)
• Quality Level: 100
• packaging: pkg of 3,600 U (11759060001 [10x360 U]); pkg of 150 U (11681834001); pkg of 720 U (11681842001 [2x360 U])
• manufacturer/tradename: Roche
• parameter: 68 °C optimum reaction temp.
• technique(s): PCR: suitable
• storage temp.: −20°C

Description

General description

Expand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture produces a high yield of PCR product from genomic DNA. Expand Long Template PCR System allows amplification of DNA fragments up to 20kb from human genomic DNA, and 40kb from λDNA.
The Expand Long Template PCR System copies DNA threefold more accurately than Taq DNA Polymerase.
Misincorporations cause Taq DNA polymerase to fall off the DNA strand. The 3′→ 5′ exonuclease activity of the proofreading polymerase reaction mix amplify DNA three times more accurately than Taq DNA polymerase alone.
Long PCR products require a special buffer and optimized protocol. This kit has three buffers for amplifying fragments from 0.5 to 9 kb, 9 to 12 kb, and larger than 12kb. For high template quality, use Roche′s High Pure Template Preparation Kit to prepare genomic DNA template.

Application

The main application of the Expand Long Template PCR System is the accurate amplification of very long target sequences, even from genomic DNA. Use this optimized enzyme blend and specially developed three-buffer set to generate PCR products from 5 to 20kb in length.
Choose 1^st generation Expand Long Template PCR System for long and accurate PCR reactions. Use this optimized enzyme blend and specially developed three-buffer set to generate PCR products from 5 to 20kb in length. Use the product for:

• PCR[1]
• RT-PCR or long-range PCR[2][3]
• Large-fragment amplification
• Genome mapping and sequencing
• Contig construction
• Characterization of cloned sequences in lambda phages or cosmids
• Rapid identification and cloning of complete genes from genomic DNA  or cDNA sequence as starting material

As economical alternative we recommend to update to 2^nd generation Expand Long Range, dNTPack.
For amplicon sizes of 20 to 35kb choose Expand 20kb^PLUS PCR System, dNTPack.

Features and Benefits

Expand Long Template PCR System is ideal for genome mapping and sequencing, contig construction, characterization of cloned sequences in lambda phages or cosmids, and eukaryotic-gene cloning and analysis.

• Amplify longer templates: This 3-buffer set amplifies products from 5 to 20kb.
• Achieve higher yields and 3-fold higher fidelity compared to Taq DNA Polymerase.
• Improve your PCR: Obtaining more full-length PCR product is ideal downstream.

Packaging

1 kit containing 4 components

Quality

Each lot is PCR function-tested using human genomic DNA and specific primers for a 9kb, 12kb, and 15kb fragment. The enzyme mix is tested for the absence of any contaminating activities, including endo- or exonucleases and nicking activity according to the current Quality Control procedures.

Unit Definition

Volume Activity: 5 U/μl

Preparation Note

Working concentration: Optimal enzyme concentration varies from 0.5 to 5.0 U per 50 μl reaction. The standard enzyme concentration is 3.75 U per 50 μl reaction.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Expand is a trademark of Roche

Kit Components Only

• Expand Long Template Enzyme Mix
• Expand Long Template Buffer 1, with 17.5 mM MgCl₂ 10x concentrated
• Expand Long Template Buffer 2, with 27.5 mM MgCl₂ 10x concentrated
• Expand Long Template Buffer 3, with 27.5 mM MgCl₂ and detergents 10x concentrated

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash