- UFC5100
UFC5100
Amicon Ultra-0.5 Centrifugal Filter Unit
Ultracel-100 regenerated cellulose membrane, 0.5 mL sample volume
Recommended Products
material
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
manufacturer/tradename
Amicon® Ultra
parameter
0.5 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
pore size
100 kDa NMWCO
shipped in
ambient
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This Item | UFC5030 | UFC5003 | UFC5010 |
|---|---|---|---|
| material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| pore size 100 kDa NMWCO | pore size 30 kDa NMWCO | pore size 3 kDa NMWCO | pore size 10 kDa NMWCO |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
| parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume |
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
Application
- Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
Linkage
Physical form
Legal Information
Storage Class Code
10-13 - German Storage Class 10 to 13
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