UC0E02
UCOE® Single Expression Hygromycin Vector Set
Synonym(s):
UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element, UCOE02
About This Item
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General description
CET 1019 HS-hygro-SceI is a mammalian expression plasmid vector containing the mouse Rps3 UCOE upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.
CET 1019 AS-hygro-SceI is a mammalian expression plasmid vector containing the murine Rps3 UCOE upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.
The CET 1019 HS-hygro-SceI and CET 1019 AS-hygro-SceI vectors possess the following features:
- A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
- A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
- A hygromycin antibiotic resistance gene for selection of stably transfected mammalian cells
- A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
- An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes
THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.
Application
General Cell Biology
All
All
Components
2) 10ug CET 1019 AS-hygro-SceI (+) Vector (CS221289)
3) 10ug SC HS-hygro (-) Vector (CS221290)
4) 10ug SC AS-hygro (-) Vector (CS221291)
Storage and Stability
Other Notes
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Storage Class Code
12 - Non Combustible Liquids
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
UC0E02:
Certificates of Analysis (COA)
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