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Safety Information

SCT214

Sigma-Aldrich

BioTracker Orange-NHS Live Cell pH dye

Live Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.47

Quality Level

200

Assay

≥80% (LC)

form

lyophilized

technique(s)

cell based assay: suitable

storage temp.

−20°C

General description

BioTracker Orange-NHS Live Cell pH dye is a chemical probe which reversibly fluoresces upon acidic pH. The probe is modified with N-hydroxysuccimide ester (NHS) which forms a covalent bond with primary amines just by mixing. It can be used for labeling proteins, antibodies and other molecules. For example, it can be applied for visualizing endocytosis of target protein on cell membrane by labeling antibodies that binds to the target protein. This probe binds to primary amines just by mixing. Thus it can be used to label antibodies and other proteins which have lysine residues. Its fluorescence intensity increases ~20-fold at an acidic pH.

Spectral Properties
Absorbance (max): 544 nm
Emission (max): 565 nm (Orange)

Application

Live Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells.
  • Cell Based Assays
  • Antibody Labeling

Packaging

1 mg

Physical form

Lyophilized

Storage and Stability

Store BioTracker Orange-NHS Live Cell pH Dye at -20°C, desiccate and protect from light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCT214:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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