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SCM018

Sigma-Aldrich

Embryoid Body (EB) Formation Medium

The Embryoid Body Formation Medium (EB Media) has been optimized & qualified to support the formation of embryoid body.

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

100

form

liquid

technique(s)

cell differentiation: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

shipped in

dry ice

General description

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Most protocols used to differentiate murine ES cells to a variety of cells involve an initial step in which the spontaneous differentiation of ES cells proceed through the formation of embryoid body (EB). EBs are formed by culturing ES cells in suspension culture in low attachment Petri dishes. The non-adhesive surfaces of Petri dishes promote the aggregation of ES cells into a tight ball of cells termed embryoid body. EBs are thought to help maintain an embryonic organization and closely mimic some of the earliest stages of embryonic development.
The Embryoid Body (EB) Formation Medium has been optimized and qualified to support the formation of embryoid body. The medium can be used to form EB from hanging drops or suspension culture on non-adhesive Petri dishes. It has been validated for the differentiation of mouse ES cells into neural, adipogenic and cardiomyogenic cell lineages.

Physical form

Embryoid Body (EB) Formation Medium is a proprietary formulation that contains fetal bovine serum.

Storage and Stability

Stored at -20°C until ready to use. Upon thawing, store at 2-8°C for up to one month.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCM018:

Certificates of Analysis (COA)

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Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

rganoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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