Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

SCC284

Sigma-Aldrich

EpH4 Mouse Mammary Epithelial Cell Line

EpH4 mouse mammary epithelial cell line is a versatile system for studying a spectrum of morphological transformation spanning breast cancer metastasis, EMT, and hormonal regulation.

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

application(s)

cell analysis

General description

Mammary epithelial cells are a model for cellular plasticity, the ability to undergo varied morphological changes. One of the significant aspects of mammary-derived tumors is epithelial-to-mesenchymal transition (EMT), which gives rise to the migratory behavior required for metastasis (1). Cellular plasticity makes mammary epithelial cell models valuable for the study of breast cancer malignancy in addition to hormonal regulation of differentiation and epithelial cell polarization.

Eph4 is a spontaneously immortalized cell line derived from primary murine mammary epithelial cells (2). EpH4 cells can be induced to synthesize milk proteins, such as caseins, upon the induction with lactogenic hormones such as prolactin, hydrocortisone, and insulin. For normal maintenance Eph4 cells should be cultured at high density (semi-confluency) and passaged before reaching confluency to avoid polarization and selective culture of single cells. Post-confluent Eph4 cells undergo polarization and formation of hemicysts or dome-like structures characterized by unidirectional transport of ions (3).

<bold>Source:</bold>
Eph4 cell line is a spontaneously immortalized clone derived from primary mammary epithelial cells of a female BALB/c mouse.

Research Category:
Cancer

Cell Line Origin

Mouse, Epithelial Cells

Packaging

&ge;1X106 cells/vial

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCC284:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service